高效凝胶过滤色谱法测定菊糖聚合度

doi:10.3976/j.issn.1002-4026.2020.04.001

山东科学 ›› 2020, Vol. 33 ›› Issue (4): 1-6.doi: 10.3976/j.issn.1002-4026.2020.04.001

• 中药与天然活性产物 • 下一篇

高效凝胶过滤色谱法测定菊糖聚合度

李玲玉¹，李雨晨²，左兆河³，田宝兰³，郑振佳^1*

1. 山东农业大学食品科学与工程学院山东省高校食品加工技术与质量控制重点实验室, 山东泰安 271018；2. 山东医药技师学院，山东泰安 271000；3. 山东植益源健康科技有限公司，山东泰安 271000

收稿日期:2020-02-05 出版日期:2020-07-27 发布日期:2020-07-27
通信作者: 郑振佳，博士，副教授，研究方向为农产品加工与贮藏研究。Tel:18001301303，E-mail: zhengzhenjia@sdau.edu.cn
作者简介:李玲玉（1996—），硕士研究生，研究方向为农产品加工与贮藏研究。E-mail: sdnydxlly@163.com
基金资助:
烟台市重点研发计划（2018ZDCX014）；山东省农业重大应用技术创新项目；莱阳市新旧动能转换科技创新计划

Development of high performance gel chromatography for the determination of inulin polymerization

LI Ling-yu¹，LI Yu-chen², ZUO Zhao-he³，TIAN bao-lan³，ZHENG Zhen-jia^1*

1. Key Laboratory of Food Processing Technology and Quality Control in Shandong Province,College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China; 2. Shandong Medicine Technician College, Taian 271000, China; 3. Shandong Zhiyiyuan Health Technology Co., Ltd., Taian 271000, China

Received:2020-02-05 Online:2020-07-27 Published:2020-07-27

摘要/Abstract

摘要： 菊糖属于天然大分子多糖，其生理活性与性质受聚合度的影响。以OHpak SB-802.5 HQ为色谱柱，进样量为20 μL，柱温为30 ℃，流动相为0.3 mol/L NaNO3溶液，流速为0.3 mL/min，通过GPC 软件拟合三次方程标准曲线，利用高效凝胶色谱方法检测了9个样品的分子量并计算了其聚合度。结果表明，9个样品的菊糖聚合度在10~29，同种原材料的不同批次和不同品种间聚合度均存在差异。该方法为分析样品中菊糖的结构性质及控制产品质量提供了参考依据。

关键词: 菊糖, 聚合度, 高效凝胶过滤色谱, 多糖含量, 相对分子量

Abstract: Inulin is a type of natural macromolecular polysaccharide and its physiological activity and processing characteristics are affected by the degree of polymerization.In this study,OHpak SB-802.5 HQ was used as the chromatographic column.The injection volume was 20 μL,the column temperature was 30 ℃,the mobile phase was 0.3 moL/L NaNO₃ solution,and the flow rate was 0.3 mL/min.The cubic equation curve was fitted using GPC software.The molecular weights of nine samples were measured using high-performance gel chromatography and the degree of polymerization was calculated. Results showed that the inulin polymerization degree of inulin of nine samples ranged between 10 and 29 and the polymerization degree of different batches and varieties of the same raw material were different. The proposed method provides a reference for analyzing the structural properties and controlling the product quality of inulin.

Key words: inulin, degree of polymerization, high performance gel filtration chromatography, polysaccharide content, relative molecular weight

中图分类号:

O657.7

李玲玉, 李雨晨, 左兆河, 田宝兰, 郑振佳. 高效凝胶过滤色谱法测定菊糖聚合度[J]. 山东科学, 2020, 33(4): 1-6.

LI Ling-yu, LI Yu-chen, ZUO Zhao-he, TIAN bao-lan, ZHENG Zhen-jia. Development of high performance gel chromatography for the determination of inulin polymerization[J]. Shandong Science, 2020, 33(4): 1-6.

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高效凝胶过滤色谱法测定菊糖聚合度

Development of high performance gel chromatography for the determination of inulin polymerization

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