山东科学 ›› 2017, Vol. 30 ›› Issue (1): 20-25.doi: 10.3976/j.issn.1002-4026.2017.01.004

• 中药与天然活性产物 • 上一篇    下一篇

忍冬藤可溶性膳食纤维提取及性能分析

郭凤倩,黄祖顺,朱宝磊,刘谦*   

  1. 山东中医药大学药学院,山东 济南 250355
  • 收稿日期:2016-04-26 出版日期:2017-02-20 发布日期:2017-02-20
  • 通信作者: 刘谦(1982—),女,讲师,研究方向为中药资源与质量控制。
  • 作者简介:郭凤倩(1993—),女,研究方向为中药资源与质量控制。
  • 基金资助:

    山东省科技发展计划(2014GSF11903);2015年度大学生研究训练计划(SRT)(201510441023)

Extraction and property analysis of soluble dietary fiber from stems of Lonicera japonica

GUO Feng-qian, HUANG Zu-shun, ZHU Bao-lei, LIU Qian*   

  1. Shandong University of Traditional Chinese Medicine, Jinan 250355, China
  • Received:2016-04-26 Online:2017-02-20 Published:2017-02-20

摘要:

探讨纤维素酶法提取忍冬藤中水溶性纤维素的最佳工艺并对其性能进行分析。在单因素试验基础上,采用正交试验对提取工艺进行优化得到最佳工艺条件组合为pH=4.8,温度45 ℃,料液比1:12(g/mL),纤维素酶体积分数1.2%,提取时间6 h,浸提3次。在此条件下,水溶性膳食纤维的提取率为3.57%,所提取的水溶性纤维素持水力为6.1 g/g,膨胀力为5.3 mL/g,持油力为4.53 g/g,阳离子交换能力为0.58 mmol/g,DPPH自由基最大清除率为80.13%,具有较好的理化性能。研究认为,纤维素酶解法可用于提取忍冬藤中的水溶性膳食纤维。

关键词: 水溶性膳食纤维, 忍冬藤, 纤维素酶, 性能

Abstract:

The optimal technology of extracting soluble dietary fiber (SDF) from stems of Lonicera japonica by cellulose method was investigated and the properties of SDF were also analyzed. Based on singlefactor experiments, orthogonal experiments were performed to optimize the extractive conditions, and the results showed the optimum conditions were pH 4.8, at 45 ℃, 1∶12(g/mL)of material and water, cellulase 1.2% by volume, extracting for 6 h, 3 times.Under the above conditions, the yield of SDF was 3.57%, whose water holding capacity was 6.1 g/g, expansion force was 5.3 mL/g, oil holding capacity was 4.53 g/g, cation exchange capacity was 0.58 mmol/g, highest free radical scavenging rate on DPPH was 80.13%, and the SDF had excellent physical and chemical properties. The study indicated that the cellulose method can be used to extracting SDF from stems of Lonicera japonica.

Key words: stems of Lonicera japonica, cellulase, water soluble dietary fiber, properties

中图分类号: 

  • R284.2