生物酶对五氯联苯降解的初步研究

doi:10.3976/j.issn.1002-4026.2016.06.015

山东科学 ›› 2016, Vol. 29 ›› Issue (6): 94-97.doi: 10.3976/j.issn.1002-4026.2016.06.015

生物酶对五氯联苯降解的初步研究

季蕾，陈贯虹，傅晓文，黄玉杰，王加宁，张强^*

山东省科学院生态研究所, 山东省应用微生物重点实验室, 山东济南 250014

收稿日期:2016-08-08 出版日期:2016-12-20 发布日期:2016-12-20
作者简介:季蕾（1985—），女，博士，研究方向为生态修复。
基金资助:
国家国际科技合作专项（2014DFE90100）；山东省自然科学基金（ZR2015YL008, BS2015HZ011）

Pilot study on bioenzyme degradation to pentachlorodiphenyl

JI Lei, CHEN Guan-hong, FU Xiao-wen, HUAGN Yu-jie, WANG Jia-ning, ZHANG Qiang

Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Shandong Academy of Sciences, Jinan 250014, China

Received:2016-08-08 Online:2016-12-20 Published:2016-12-20

摘要/Abstract

摘要：

多氯联苯（PCBs）是具有代表性的持久性有机污染物（POPs），其氯代数目越多，降解越难。本文以分离筛选的6株PCBs降解细菌为材料，制备了含有NADH辅酶再生系统关键酶甲酸脱氢酶（FDH）的PCBs降解复合酶，研究了其对PCBs的降解效果。结果表明，Pseudomonas sp. MGB11胞内酶/FDH复合酶对五氯联苯PCB114的降解率在10 h内可达69.4%，对于高毒性、难降解的高氯PCBs的污染修复具有应用价值。

关键词: 降解复合酶, 五氯联苯, 甲酸脱氢酶

Abstract:

Polychlorinated biphenyls (PCBs) is a representative of persistent organic pollutants (POPs). More its chlorinated number leads to more difficult its degradation . We prepared PCBs compound degrading enzyme of key enzyme formate dehydrogenase (FDH) of NADH coenzyme regeneration system with isolated 6 PCBs degradation bacteria strains as materials. We address its degrading effect to PCBs. Results indicate that degradation rate of Pseudomonas sp. MGB11/FDH compound enzyme to pentachlorodiphenyl PCB114 is up to 69.4% within 10 h. It therefore has application value in the repair for high chlorine PCBs pollutants with high toxicity and low degradability.

Key words: pentachlorodiphenyl, formate dehydrogenase, compound degrading enzyme

中图分类号:

季蕾，陈贯虹，傅晓文，黄玉杰，王加宁，张强. 生物酶对五氯联苯降解的初步研究[J]. 山东科学, 2016, 29(6): 94-97.

JI Lei, CHEN Guan-hong, FU Xiao-wen, HUAGN Yu-jie, WANG Jia-ning, ZHANG Qiang. Pilot study on bioenzyme degradation to pentachlorodiphenyl[J]. SHANDONG SCIENCE, 2016, 29(6): 94-97.

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生物酶对五氯联苯降解的初步研究

Pilot study on bioenzyme degradation to pentachlorodiphenyl

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