基于PI3K/AKT/Gsk-3β通路研究山药多糖对运动疲劳小鼠的影响

doi:10.3976/j.issn.1002-4026.20240034

山东科学 ›› 2024, Vol. 37 ›› Issue (6): 51-58.doi: 10.3976/j.issn.1002-4026.20240034

基于PI3K/AKT/Gsk-3β通路研究山药多糖对运动疲劳小鼠的影响

曾婷¹(), 杨然¹, 李庆军¹, 宫亚楠², 王书珍¹^,³^,^*(), 刘谦¹^,^*()

1.山东中医药大学药学院,山东济南 250355
2.济南市章丘区人民医院心内一科,山东济南 250299
3.黄冈师范学院生物与农业资源学院,湖北黄冈 438000

收稿日期:2024-03-06 出版日期:2024-12-20 发布日期:2024-12-05
通信作者: *刘谦,女,教授,博士,研究方向为中药资源与可持续利用研究。E-mail:cleanlq@163.com, Tel:13290326687; 王书珍,女,博士,副教授,研究方向为中草药生物活性成分作用机制及活性成分鉴定研究。E-mail:wangshuzhen710@whu.edu.cn, Tel:18171758890
作者简介:曾婷(1998—),女,硕士研究生,研究方向为中草药生物活性成分作用机制及活性成分鉴定研究。E-mail:2452280058@qq.com
基金资助:
山东省重点研发计划项目(2021CXGC010510);财政部和农业农村部:国家现代农业产业技术体系项目(CARS-21);国家自然科学基金项目(82003892);泰山学者工程专项经费资助项目(tsqn202306187);中央引导地方科技发展资金项目(XZ202301YD0023C)

Effects of Dioscorea opposita Thunb. polysacchariades on exercise-related fatigue in mice through the PI3K/AKT/Gsk-3β pathway

ZENG Ting¹(), YANG Ran¹, LI Qingjun¹, GONG Yanan², WANG Shuzhen¹^,³^,^*(), LIU Qian¹^,^*()

1. College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
2. The First Department of Cardiology, Jinan Zhangqiu District People's Hospital, Jinan 250299, China
3. College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang 438000, China

Received:2024-03-06 Online:2024-12-20 Published:2024-12-05

摘要/Abstract

摘要：

研究山药多糖(Dioscorea opposita Thunb. polysaccharides,DTPs)对运动疲劳小鼠的抗疲劳作用及作用机制。采用力竭游泳结合疲劳转棒方法建立小鼠运动疲劳模型,分析小鼠力竭游泳时间和体重变化、疲劳代谢产物积累、相关酶活力水平、肝脏组织结构和肌肝糖原变化。结果表明:山药多糖能显著延长小鼠力竭游泳时间,减轻小鼠体重下降和肝脏损伤,显著降低血清乳酸(LD)、尿素氮(BUN)、乳酸脱氢酶(LDH)含量并显著提高超氧化物歧化酶(SOD)活力及肌糖原(MuG)和肝糖原(LG)含量,同时显著降低谷草转氨酶(AST)水平;此外,给药组显著上调骨骼肌中磷脂酰肌醇3-激酶(Phosphoinositide 3-kinase,PI3K)mRNA表达、显著降低蛋白激酶B(Protein kinase B,AKT)和糖原合成酶激酶-3(Glycogen synthase kinase-3 beta,Gsk-3β)的表达。综上,山药多糖能减少代谢产物积累、通过调节PI3K/AKT/Gsk-3β通路促进糖原合成来改善运动疲劳。

关键词: 山药多糖, 抗运动疲劳, 力竭游泳, PI3K/AKT/Gsk-3β通路

Abstract:

This study aimed to investigate the antifatigue effects and mechanisms of Dioscorea opposita Thunb. polysaccharides (DTPs) on exercise-related fatigue in mice. In a mouse model of exercise fatigue where mice were subjected to exhaustive swimming and fatigue rotarod, changes in exhaustive swimming duration, body weight, fatigue metabolite accumulation, activity levels of related enzymes, liver tissue structure, and muscle glycogen (MuG) and liver glycogen (LG) contents were assessed. The results demonstrated that DTPs significantly prolonged the exhaustive swimming duration and reduced body weight loss, hepatic damage, and serum lactic acid (LD), blood urea nitrogen (BUN), and lactate dehydrogenase (LDH) levels in the mice. DTPs also significantly enhanced superoxide dismutase (SOD) activity and MuG and LG contents and significantly reduced aspartate aminotransferase (AST) levels. Additionally, in mice administered DTPs, the mRNA expression of phosphoinositide 3-kinase (PI3K) was significantly upregulated, whereas that of protein kinase B (AKT) and glycogen synthase kinase-3 (Gsk-3β) was significantly downregulated in skeletal muscle. Taken together, DTPs can reduce metabolite accumulation and ameliorate exercise fatigue by regulating the PI3K/AKT/Gsk-3β pathway to promote glycogen synthesis.

Key words: Dioscorea opposita Thunb. polysaccharides, anti-exercise fatigue, exhaustive swimming, PI3K/AKT/Gsk-3β pathway

中图分类号:

曾婷, 杨然, 李庆军, 宫亚楠, 王书珍, 刘谦. 基于PI3K/AKT/Gsk-3β通路研究山药多糖对运动疲劳小鼠的影响[J]. 山东科学, 2024, 37(6): 51-58.

ZENG Ting, YANG Ran, LI Qingjun, GONG Yanan, WANG Shuzhen, LIU Qian. Effects of Dioscorea opposita Thunb. polysacchariades on exercise-related fatigue in mice through the PI3K/AKT/Gsk-3β pathway[J]. Shandong Science, 2024, 37(6): 51-58.

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参考文献 28

[1]	马丽, 马英. 响应面法优化辣木籽多肽饮料配方及其抗运动疲劳作用的研究[J]. 保鲜与加工, 2023, 23(12): 38-46. DOI: 10.3969/j.issn.1009-6221.2023.12.005.
[2]	ZHANG X L, REN F, HUANG W, et al. Anti-fatigue activity of extracts of stem bark from Acanthopanax senticosus[J]. Molecules, 2010, 16(1): 28-37. DOI: 10.3390/molecules16010028.
[3]	SUPRUNIUK E, GÓRSKI J, CHABOWSKI A. Endogenous and exogenous antioxidants in skeletal muscle fatigue development during exercise[J]. Antioxidants, 2023, 12(2): 501. DOI: 10.3390/antiox12020501.
[4]	LUO C H, XU X R, WEI X C, et al. Natural medicines for the treatment of fatigue: bioactive components, pharmacology, and mechanisms[J]. Pharmacological Research, 2019, 148: 104409. DOI: 10.1016/j.phrs.2019.104409.
[5]	PENG Z Y, HU X L, LI X S, et al. Protective effects of cyanidin-3-O-glucoside on UVB-induced chronic skin photodamage in mice via alleviating oxidative damage and anti-inflammation[J]. Food Frontiers, 2020, 1(3): 213-223. DOI: 10.1002/fft2.26.
[6]	李慧敏, 贺凯, 郑慧, 等. 中医药健脾的保健作用机制及药食资源[J]. 中草药, 2020, 51(3): 780-787. DOI: 10.7501/j.issn.0253-2670.2020.03.031.
[7]	王丹, 高永欣, 冯小雨, 等. 山药对小鼠体力疲劳缓解及抗氧化作用的研究[J]. 河南工业大学学报(自然科学版), 2016, 37(1): 88-94. DOI: 10.16433/j.cnki.issn1673-2383.2016.01.016.
[8]	周庆峰, 姜书纳, 马亢, 等. 铁棍山药多糖抗疲劳及耐缺氧作用研究[J]. 时珍国医国药, 2014, 25(2): 284-285. DOI: 10.3969/j.issn.1008-0805.2014.02.012.
[9]	WANG Y, LIU Y X, ZHANG Y Q, et al. Effects of the polysaccharides extracted from Chinese yam (Dioscorea opposita Thunb.) on cancer-related fatigue in mice[J]. Food & Function, 2021, 12(21): 10602-10614. DOI: 10.1039/d1fo00375e.
[10]	崔恺, 管雅琪, 张钊, 等. 潞党参通过PI3K/AKT/GSK3β通路缓解运动性疲劳的作用机制研究[J]. 中药材, 2022, 45(10): 2471-2475. DOI: 10.13863/j.issn1001-4454.2022.10.034.
[11]	王祥山, 朱映黎, 吕美豫, 等. 鹿鞭对肾阴虚及肾阳虚小鼠抗疲劳作用及PI3K-Akt通路机制研究[J]. 中国中药杂志, 2023, 48(11): 3032-3038. DOI: 10.19540/j.cnki.cjcmm.20230202.401.
[12]	叶美芝, 伍紫仪, 吴金松, 等. 山药皮多糖的分离纯化、结构特征及体外抗氧化活性研究[J]. 食品工业科技, 2023, 44(19): 78-85. DOI: 10.13386/j.issn1002-0306.2022120074.
[13]	徐俊杰, 王珺, 杨占群. 山药多糖对低强度连续微波辐射小鼠免疫系统功能的影响[J]. 上海中医药杂志, 2019, 53(11): 82-85. DOI: 10.16305/j.1007-1334.2019.11.017.
[14]	ZHANG X Y, JING S, LIN H J, et al. Anti-fatigue effect of anwulignan via the NRF2 and PGC-1α signaling pathway in mice[J]. Food & Function, 2019, 10(12): 7755-7766. DOI: 10.1039/c9fo01182j.
[15]	XIE Q, SUN Y T, CAO L L, et al. Antifatigue and antihypoxia activities of oligosaccharides and polysaccharides from Codonopsis pilosula in mice[J]. Food & Function, 2020, 11(7): 6352-6362. DOI: 10.1039/d0fo00468e.
[16]	胡杨, 赵勉, 邱雨轩, 等. 药食同源中药铁皮石斛的研究进展[J]. 南京中医药大学学报, 2024, 40(1): 94-108. DOI: 10.14148/j.issn.1672-0482.2024.0094.
[17]	嵇晶, 张云羽, 王令充, 等. 基于经典名方的药食同源大健康产品开发应用探讨[J]. 南京中医药大学学报, 2023, 39(9): 961-966. DOI: 10.14148/j.issn.1672-0482.2023.0961.
[18]	陈仪新, 林翠英, 黄昕哲, 等. 复方四神蒸糕的研制[J]. 现代食品, 2023, 29(17): 88-91. DOI: 10.16736/j.cnki.cn41-1434/ts.2023.17.022.
[19]	HSU Y J, HUANG W C, CHIU C C, et al. Capsaicin supplementation reduces physical fatigue and improves exercise performance in mice[J]. Nutrients, 2016, 8(10): 648. DOI: 10.3390/nu8100648.
[20]	ZHANG C J, GUO J Y, CHENG H, et al. Spatial structure and anti-fatigue of polysaccharide from Inonotus obliquus[J]. International Journal of Biological Macromolecules, 2020, 151: 855-860. DOI: 10.1016/j.ijbiomac.2020.02.147.
[21]	CHEN X W, ZHANG J, LI H, et al. A comprehensive comparison of different selenium supplements: mitigation of heat stress and exercise fatigue-induced liver injury[J]. Frontiers in Nutrition, 2022, 9: 917349. DOI: 10.3389/fnut.2022.917349.
[22]	LI M, KONG Y D, LI L, et al. Effects of dietary carbohydrate sources on growth performance, immunity and glycometabolic enzyme activity of Rhynchocypris lagowskii Dybowski[J]. Aquaculture Research, 2021, 52(1): 323-333. DOI: 10.1111/are.14895.
[23]	FIORENZA M, HOSTRUP M, GUNNARSSON T P, et al. Neuromuscular fatigue and metabolism during high-intensity intermittent exercise[J]. Medicine and Science in Sports and Exercise, 2019, 51(8): 1642-1652. DOI: 10.1249/MSS.0000000000001959.
[24]	NIPATE S S, TIWARI A H. Antioxidant and immunomodulatory properties of Spilanthes oleracea with potential effect in chronic fatigue syndrome infirmity[J]. Journal of Ayurveda and Integrative Medicine, 2020, 11(2): 124-130. DOI: 10.1016/j.jaim.2017.08.008.
[25]	徐小珊, 马伟, 熊罗节, 等. 隔药饼灸对慢性疲劳综合征大鼠血乳酸及AMPK/PGC-1α信号通路的影响[J]. 针刺研究, 2022, 47(10): 878-884. DOI: 10.13702/j.1000-0607.20220017.
[26]	代紫阳, 董玉山, 李继安, 等. 施今墨对药配方对2型糖尿病大鼠肝脏胰岛素抵抗及PI3K/AKT/GSK-3β信号通路的影响[J]. 山东医药, 2020, 60(14): 35-38. DOI: 10.3969/j.issn.1002-266X.2020.14.010.
[27]	JERE S W, HOURELD N N, ABRAHAMSE H. Role of the PI3K/AKT (mTOR and GSK3β) signalling pathway and photobiomodulation in diabetic wound healing[J]. Cytokine & Growth Factor Reviews, 2019, 50: 52-59. DOI: 10.1016/j.cytogfr.2019.03.001.
[28]	YU J C, JIANG W H, WANG S Y, et al. Microencapsulated hawthorn berry polyphenols alleviate exercise fatigue in mice by regulating AMPK signaling pathway and balancing intestinal microflora[J]. Journal of Functional Foods, 2022, 97: 105255. DOI: 10.1016/j.jff.2022.105255.

基因	上游(5'-3')	下游(5'-3')
PI3K	GGGAGCAGCAACCGAAACAAAG	CCACTACGGAGCAGGCATAGC
AKT	GCCACGCTACTTCCTCCTCAAG	GACTCTCGCTGATCCACATCCTG
Gsk-3β	TGTGTTGGCTGAATTGTTGCTAGG	AATTTGCTCCCTTGTTGGTGTTCC
β-actin	GCGCAAGTACTCTGTGTGGA	CATCGTACTCCTGCTTGCTG

组别	肝细胞水样性变性	肝细胞坏死	免疫细胞浸润
CG	少量	无	无
MG	弥漫性	少量	淋巴细胞
DTP-L	弥漫性	无	无
DTP-H	大量	无	无
PG	多量	无	无

基于PI3K/AKT/Gsk-3β通路研究山药多糖对运动疲劳小鼠的影响

Effects of Dioscorea opposita Thunb. polysacchariades on exercise-related fatigue in mice through the PI3K/AKT/Gsk-3β pathway

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