UHPLC-MS/MS法同时测定大鼠血浆中汉黄芩素、隐绿原酸和白杨素-7-O-葡萄糖醛酸苷含量

doi:10.3976/j.issn.1002-4026.2018.02.003

山东科学 ›› 2018, Vol. 31 ›› Issue (2): 14-20.doi: 10.3976/j.issn.1002-4026.2018.02.003

UHPLC-MS/MS法同时测定大鼠血浆中汉黄芩素、隐绿原酸和白杨素-7-O-葡萄糖醛酸苷含量

李翠^1,2，罗孟雄³，聂其霞²，臧琛²，王国华²，罗赣^1,2，张保献²*

1.中国中医科学院,北京 100700;2.中国中医科学院中药研究所雾化吸入制剂研究中心,北京 100700;3.山东中医药大学，山东济南 250355

收稿日期:2017-10-09 出版日期:2018-04-20 发布日期:2018-04-20
通信作者: 张保献，男，研究员，博士生导师，研究方向为中药新药研发。E-mail：zhangbaoxian925@tom.com E-mail:zhangbaoxian925@tom.com
作者简介:李翠（1982—），女，博士，研究方向为中药新药研发。E-mail：licui-1982@163.com
基金资助:
全国中药特色技术传承人才培训项目（L2016002）

Simultaneous determination of wogonin, 4-dicaffeoylquinic acid and chrysin-7-O-Beta-D-glucoronide in rats plasma by UHPLC-MS/MS

LI Cui^1,2, LUO Meng-xiong³, NIE Qi-xia², ZANG Chen², WANG Guo-hua², LUO Gan^1,2, ZHANG Bao-xian²*

1. China Academy of Chinese Medical Science, Beijing 100700, China; 2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing 100700, China; 3. Shandong University of Traditional Chinese Medicine, Jinan 250355, China

Received:2017-10-09 Online:2018-04-20 Published:2018-04-20

摘要/Abstract

摘要：

建立了UHPLC-MS/MS同时测定大鼠血浆中的汉黄芩素、隐绿原酸和白杨素-7-O-葡萄糖醛酸苷含量的方法。以芹菜苷为内标，采用ACQUITY UPLC HSS T3色谱柱(2.1 mm×100 mm, 1.8 μm)，乙腈和0.1％甲酸溶液为流动相，流速为0.2 mL·min^-1。结果表明，汉黄芩素、隐绿原酸和白杨素-7-O-葡萄糖醛酸苷线性范围为1～1 000 ng·mL^-1(R2>0.99)，最低定量浓度为1 ng·mL^-1，提取回收率、基质效应均符合生物样品分析要求。建立的方法灵敏、准确，可用于大鼠血浆中汉黄芩素、隐绿原酸和白杨素-7-O-葡萄糖醛酸苷的测定及其药代动力学研究。

关键词: 大鼠血浆, 白杨素-7-O-葡萄糖醛酸苷, 汉黄芩素, UHPLC-MS/MS, 隐绿原酸

Abstract:

A rapid ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method was developed for the simultaneous analysis of wogonin, 4dicaffeoylquinic acid and chrysin-7-O-Beta-D-glucoronide in rats plasma. Apiin was selected as the internal standard. The analysis was carried out on an ACQUITY UPLC HSS T3 column (2.1 mm×100 mm, 1.8 μm), with acetonitrile and 0.1% formic acid aqueous solution, gradient elution. The flow rate was 0.2 mL·min^-1. Linear responses were obtained for wogonin, 4-dicaffeoylquinic acid and chrysin-7-O-Beta-D-glucoronide ranging from 1 to 1 000 ng·mL^-1. The correlation coefficients were better than 0.99. Both recovery rates and matrix effects met the requirement of biological sample analysis. The established UHPLC-MS/MS quantitative analysis method shows high sensitivity, accuracy and satisfying recovery, and can be utilized to determine the pharmacokinetic data of wogonin, 4-dicaffeoylquinic acid and chrysin-7-O-Beta-D-glucoronide in rats plasma.

Key words: rats plasma, 4-dicaffeoylquinic acid, wogonin, chrysin-7-O-Beta-D-glucoronide, UHPLC-MS/MS

中图分类号:

R284.1

李翠，罗孟雄，聂其霞，臧琛，王国华，罗赣，张保献. UHPLC-MS/MS法同时测定大鼠血浆中汉黄芩素、隐绿原酸和白杨素-7-O-葡萄糖醛酸苷含量[J]. 山东科学, 2018, 31(2): 14-20.

LI Cui, LUO Meng-xiong, NIE Qi-xia, ZANG Chen, WANG Guo-hua, LUO Gan, ZHANG Bao-xian. Simultaneous determination of wogonin, 4-dicaffeoylquinic acid and chrysin-7-O-Beta-D-glucoronide in rats plasma by UHPLC-MS/MS[J]. SHANDONG SCIENCE, 2018, 31(2): 14-20.

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UHPLC-MS/MS法同时测定大鼠血浆中汉黄芩素、隐绿原酸和白杨素-7-O-葡萄糖醛酸苷含量

Simultaneous determination of wogonin, 4-dicaffeoylquinic acid and chrysin-7-O-Beta-D-glucoronide in rats plasma by UHPLC-MS/MS

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