知母-黄柏配伍抗炎作用机制的网络药理学研究

doi:10.3976/j.issn.1002-4026.2021.06.006

摘要/Abstract

摘要：

知母-黄柏配伍是临床上常用的“相须”配伍药对,具有抗炎作用,但其机制研究不足。本研究采用网络药理学方法研究知母-黄柏药对的抗炎机制。结果表明,知母-黄柏药对有182个潜在抗炎靶点;知母、黄柏、炎症三者共有靶点受调控分大于等于2倍中位数的靶点有29个,如PTGS2、PTGS1、RXRA、ADRB2、AR、CHRM1、ADRA1B、CHRM3、F10、NOS3等,这些靶点都与炎症的生物过程密切相关。抗炎靶点GO(gene ontology)分析涉及RNA聚合酶II启动子转录的调控、细胞增殖调节、细胞凋亡的调节、一氧化氮生物合成调节等;KEGG(Kyoto encyclopedia of genes and genomes)通路分析涉及TNF信号通路、HIF-1信号通路、PI3K-Akt信号通路、FOXO信号通路、VEGF信号通路、T细胞受体信号通路、Toll样受体信号通路、胰岛素抵抗等。研究提示知母-黄柏“相须”配伍的分子机制是通过共同调控信号通路发挥抗炎作用, 为其临床使用提供了科学依据。

关键词: 知母, 黄柏, 网络药理学, 炎症, 信号通路

Abstract:

The combination of Anemarrhenae Rhizoma-Rhellodendri Chinensis Cortex is a commonly used combinatorial medicine in clinical practice, which belongs to the “mutual promotion”. Anemarrhenae Rhizoma-Rhellodendri Chinensis Cortex combination has an anti-inflammatory effect, but the mechanism underlying this effect has not been studied well. In this study, the anti-inflammatory mechanism of Anemarrhenae Rhizoma-Rhellodendri Chinensis Cortex combination was studied using network pharmacology. The results showed that Anemarrhenae Rhizoma-Rhellodendri Chinensis Cortex combination has 182 potential anti-inflammatory targets, of which 29 showed regulatory scores greater than or equal to twice the median value of the common targets of Anemarrhenae Rhizoma, Rhellodendri Chinensis Cortex, and inflammation-PTGS2, PTGS1, RXRA, ADRB2, AR, CHRM1, ADRA1B, CHRM3, F10, NOS3, etc. These targets are closely related to the mechanism of inflammation. GO(gene ontology) function showed that anti-inflammatory targets were involved in RNA polymerase Ⅱ promoter transcriptional regulation, cell proliferation, apoptosis regulation, nitric oxide biosynthesis regulation, etc. The KEGG(Kyoto encyclopedia of genes and genomes) pathway was involved in TNF, HIF-1, PI3K-Akt, FOXO, VEGF, T cell receptor, and Toll-like receptor signaling pathways and insulin resistance pathway, among others. The results suggested that the molecular mechanism of Anemarrhenae Rhizoma-Rhellodendri Chinensis Cortex combination would be the coregulation of various signal pathways, exerting synergistic anti-inflammatory effects. The molecular mechanisms of the effect of Anemarrhenae Rhizoma-Rhellodendri Chinensis Cortex combination studied using network pharmacology provide a scientific basis for its clinical use.

Key words: Anemarrhenae Rhizoma, Rhellodendri Chinensis Cortex, network pharmacology, inflammation, signaling pathways

中图分类号:

R285

潘会君,朱全刚,朱聪聪. 知母-黄柏配伍抗炎作用机制的网络药理学研究[J]. 山东科学, 2021, 34(6): 41-50.

PAN Hui-jun,ZHU Quan-gang,ZHU Cong-cong. Network pharmacology study on the anti-inflammatory mechanism of Anemarrhenae Rhizoma-Rhellodendri Chinensis Cortex combination[J]. Shandong Science, 2021, 34(6): 41-50.

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