射干-麻黄配伍治疗咳嗽变异性哮喘的分子机制探索

doi:10.3976/j.issn.1002-4026.2021.05.004

山东科学 ›› 2021, Vol. 34 ›› Issue (5): 26-33.doi: 10.3976/j.issn.1002-4026.2021.05.004

射干-麻黄配伍治疗咳嗽变异性哮喘的分子机制探索

梅伟英，陆炜青*，陈韫炜，梅文星，申春悌

常州市中医医院呼吸科，江苏常州 213000

收稿日期:2021-01-18 出版日期:2021-10-14 发布日期:2021-10-18
通信作者: 陆炜青，主任中医师，研究方向为中西医结合治疗呼吸系统疾病。E-mail：13646127527@163.com
作者简介:梅伟英（1982—），硕士，副主任中医师，研究方向为中西医结合治疗呼吸系统疾病。Tel:15995039815，E-mail：mwy-1982327@163.com
基金资助:
申春悌全国名老中医药专家传承工作室（人教函〔2018〕134号）

Investigation of the molecular mechanism of Belamcandae Rhizoma-Ephedrae Herba couple medicine for treating cough-variant asthma

MEI Wei-ying，LU Wei-qing*，CHEN Yun-wei，MEI Wen-xing，SHEN Chun-ti

Department of respiratory，Changzhou Hospital of Traditional Chinese Medicine，Changzhou 213000，China

Received:2021-01-18 Online:2021-10-14 Published:2021-10-18

摘要/Abstract

摘要： 通过网络药理学方法预测射干-麻黄配伍治疗咳嗽变异性哮喘（cough-variant asthma，CVA）的可能分子机制。采用中药系统药理学数据库与分析平台（TCMSP）筛选射干、麻黄有效活性成分及对应治疗靶点，从GeneCards获取CVA疾病靶点基因，经过映射分析获得射干-麻黄活性成分直接作用的CVA目标靶点。构建中药-活性成分-靶点基因调控网络，通过String数据库进行目标靶点的蛋白质-蛋白质相互作用分析并筛选关键基因，借助生物信息分析工具预测靶点基因参与的基因本体生物学过程、京都基因与基因组百科全书信号通路。结果表明：从TCMSP获得射干-麻黄有效活性成分30个，直接调控的CVA靶点基因75个，射干-麻黄调控CVA存在多成分、多靶点的网络调控作用；靶点基因主要富集于核受体活性、直接配体调控序列特异性DNA结合的转录因子活性、泛素样蛋白连接酶结合、甾体激素受体活性、类固醇结合等98个生物学过程，以及PI3K-Akt信号通路、肿瘤中的微小RNA、乙型肝炎、卡波西肉瘤相关疱疹病毒感染、人类巨细胞病毒感染等110条信号通路。射干-麻黄活性成分可能通过抑制气道炎症反应与高反应性、调节自身免疫功能等环节发挥治疗CVA的作用。

关键词: 射干, 麻黄, 咳嗽变异性哮喘, 网络药理学, 分子机制

Abstract: This study aimed to investigate the molecular mechanism of Belamcandae Rhizoma-Ephedrae Herba couple medicine for treating cough-variant asthma on the basis of network pharmacology. The active ingredients and targets of Belamcandae Rhizoma-Ephedrae Herba couple medicine were obtained using the traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP). The disease targets corresponding to cough-variant asthma were obtained from the GeneCards Database. The protein-protein interaction network was constructed and analyzed using the String online database. Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were conducted using R language. Results show that a total of 30 active ingredients of Belamcandae Rhizoma-Ephedrae Herba couple medicine and 75 targets of cough-variant asthma were obtained. The interaction between the ingredients and targets is close and complicated. The targets were enriched in 98 GO processes, including nuclear receptor activity, transcription factor activity, direct ligand-regulated sequence-specific DNA binding, ubiquitin-like protein ligase binding, steroid hormone receptor activity, and steroid binding. The targets were enriched in 110 KEGG pathways, including PI3K-Akt signaling pathway, microRNAs in cancer, hepatitis B, Kaposi sarcoma-associated herpesvirus infection, and human cytomegalovirus infection. Belamcandae Rhizoma-Ephedrae Herba couple medicine may play an important role in the regulation and treatment of cough-variant asthma by regulating body immunity, anti-inflammatory activity, and reducing airway hyperreactivity.

Key words: Belamcandae Rhizoma, Ephedrae Herba, cough-variant asthma, network pharmacology, molecular mechanism

中图分类号:

R259

梅伟英, 陆炜青, 陈韫炜, 梅文星, 申春悌. 射干-麻黄配伍治疗咳嗽变异性哮喘的分子机制探索[J]. 山东科学, 2021, 34(5): 26-33.

MEI Wei-ying, LU Wei-qing, CHEN Yun-wei, MEI Wen-xing, SHEN Chun-ti. Investigation of the molecular mechanism of Belamcandae Rhizoma-Ephedrae Herba couple medicine for treating cough-variant asthma[J]. Shandong Science, 2021, 34(5): 26-33.

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射干-麻黄配伍治疗咳嗽变异性哮喘的分子机制探索

Investigation of the molecular mechanism of Belamcandae Rhizoma-Ephedrae Herba couple medicine for treating cough-variant asthma

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