基于白色念珠菌体外抑菌效果的复方苦参洗剂抑菌作用评价

doi:10.3976/j.issn.1002-4026.2019.03.005

山东科学 ›› 2019, Vol. 32 ›› Issue (3): 29-35.doi: 10.3976/j.issn.1002-4026.2019.03.005

基于白色念珠菌体外抑菌效果的复方苦参洗剂抑菌作用评价

程洪杰¹，李岩^1*，孙铁峰²

1.山东省新泰市中医医院，山东泰安 271200；2.山东省中医药研究院，山东济南 250014

收稿日期:2018-12-23 出版日期:2019-06-20 发布日期:2019-06-01
通信作者: 李岩（1988—），男，硕士，主管中药师，研究方向为中药临床药学与临床药理学。E-mail：lysdzyy00@163.com E-mail:lysdzyy00@163.com
作者简介:程洪杰（1989—），女，微生物检验师，研究方向为临床微生物检验及中药抗菌研究。E-mail：15753867265@163.com
基金资助:
山东省中医药科技发展计划项目（2015-171）

Evaluation of antifungal effect of Compound Sophorae Flavescentis Radix Lotion based on antifungal effect of Candida albicans in vitro

CHENG Hong-jie¹, LI Yan^1*, SUN Tie-feng²

1. Xintai Hospital of Traditional Chinese Medicine, Taian 271200, China; 2. Shandong Academy of Chinese Medicine, Jinan 250014, China

Received:2018-12-23 Online:2019-06-20 Published:2019-06-01

摘要/Abstract

摘要： 观察复方苦参洗剂中君、臣、佐各药组联合抑菌性质以及协同增效作用。采用加热回流水提法提取各药组获得相应提取液,采用二倍稀释法测得相应各药组单用及联合对白色念珠菌的最低抑菌浓度(ρMIC)，采用联合抑菌指数（IFIC）判定两药组联合抑菌作用性质。结果显示，8味中药水提液的ρMIC范围为7.50 ~30.00 mg/mL,君、臣、佐各药组的 ρMIC范围为4.01 ~21.00 mg/mL，复方组ρMIC为2.22 mg/mL，黄柏+苦参、黄柏+白矾、花椒+白矾的IFIC≤0.5，黄柏+花椒、苦参+花椒、苦参+白矾3组0.5＜IFIC≤1；君药组+臣药组IFIC=0.375 0，君药组+佐药组IFIC=0.750 0，臣药组+佐药组IFIC=1.000 0。复方组及君药组的联合抑菌效果明显强于单味中药的抑菌效果，黄柏、苦参、花椒、白矾4味君药对白色念珠菌抑菌活性较强，复方苦参洗剂抑菌效果的增强与各药组抑菌作用的协同和相加有关。

关键词: 复方苦参洗剂, 最低抑菌浓度, 联合抑菌, 联合抑菌指数, 协同

Abstract: The combined antifungal properties and synergistic effects of monarch drug, ministerial drug and adjuvant drug of Compound Sophora Flavescens Radix lotion on Candida albicans in vitro were observed in this paper. The extract solution of each group of Compound Sophora Flavescens Radix lotion was obtained by heating backflow water extraction method, the minimal inhibitory concentration(ρMIC)of each group alone and in combination on Candida albicans was measured by double dilution method, using the fraction inhibitory concentration index(IFIC) to determine the combined effects of two traditional Chinese medicines (TCMs). Results showed that the ρMIC range of 8 kinds of TCM water extraction was 7.50~30.00 mg/mL, the ρMIC range of monarch drug, ministerial drug and adjuvant drug was 4.01~21.00 mg/mL, the ρMIC of compound group was 2.22 mg/mL. The IFIC of Phellodendri Chinensis Cortex+Sophorae Flavescentis Radix, Phellodendri Chinensis Cortex+Alumen and Zanthoxyli Pericarpium+Alumen were less than or equal to 0.5, the IFIC of Phellodendri Chinensis Cortex+Zanthoxyli Pericarpium, Sophorae Flavescentis Radix+Zanthoxyli Pericarpium and Sophorae Flavescentis Radix + Alumen were greater than 0.5 and less than or equal to 1. The IFIC of monarch drug+ministerial drug was 0.375 0, the IFIC of monarch drug+adjuvant drug was 0.750 0，the IFIC of ministerial drug+adjuvant drug was 1.000 0.The study showed that the combined antifungal effect of Compound group and monarch group were stronger than that of single herbs. The 4 kinds of monarch drug of Phellodendri Chinensis Cortex, Sophorae Flavescentis Radix, Zanthoxyli Pericarpium and Alumen have strong antifungal activity against Candida albicans. The enhancement of the antifungal effect of compound Sophorae Flavescentis Radix Lotion was related to the synergy and addition of the antifungal effect of each drug group.

Key words: Compound Sophorae Flavescentis Radix Lotion, minimal inhibitory concentration, combined antifungal, combined antifungal index, synergistic effect

中图分类号:

R285.5

程洪杰, 李岩, 孙铁峰. 基于白色念珠菌体外抑菌效果的复方苦参洗剂抑菌作用评价[J]. 山东科学, 2019, 32(3): 29-35.

CHENG Hong-jie, LI Yan, SUN Tie-feng. Evaluation of antifungal effect of Compound Sophorae Flavescentis Radix Lotion based on antifungal effect of Candida albicans in vitro[J]. Shandong Science, 2019, 32(3): 29-35.

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基于白色念珠菌体外抑菌效果的复方苦参洗剂抑菌作用评价

Evaluation of antifungal effect of Compound Sophorae Flavescentis Radix Lotion based on antifungal effect of Candida albicans in vitro

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