木霉属中国新记录种Trichoderma paratroviride

doi:10.3976/j.issn.1002-4026.2015.05.006

山东科学 ›› 2015, Vol. 28 ›› Issue (5): 35-40.doi: 10.3976/j.issn.1002-4026.2015.05.006

木霉属中国新记录种Trichoderma paratroviride

张广志, 张新建, 陈凯, 吴晓青,李纪顺，杨合同

山东省科学院生态研究所，山东省应用微生物重点实验室，山东济南 250014

收稿日期:2015-07-21 出版日期:2015-10-20 发布日期:2015-10-20
作者简介:张广志（1978-），男，助理研究员，研究方向为农业微生物资源挖掘及应用研究。
基金资助:
山东省科技发展计划 (2014GSF121028，2013GNC20119)；国家自然科学基金(31572044)；国家科技基础性工作专项(2014FY120900)

Trichoderma paratroviride,Chinese new record of Trichoderma

ZHANG Guangzhi, ZHANG Xinjian,CHEN Kai,WU Xiaoqing,LI Jishun,YANG Hetong

Shandong Provincial Key Laboratory of Applied Microbiology, Institute of Ecology, Shandong Academy of Sciences, Jinan 250014, China

Received:2015-07-21 Online:2015-10-20 Published:2015-10-20

摘要/Abstract

摘要： 从山东保护地土壤中分离得到木霉菌Y15，结合ITS、tef1α和形态学特征进行鉴定。结果发现Y15菌株为近深绿木霉（Trichoderma paratroviride）。该菌株在PDA上菌落稠密，老熟后毡状，灰绿色；分生孢子梗主轴长，着生短的二次分枝，再次分枝少,分枝成对或轮生。瓶梗通常2~4个轮状排列，烧瓶形或锥形，直或弯曲；分生孢子亚球形至卵圆形，绿色，光滑。tef1α序列与近深绿木霉模式菌株S489和S385序列同源性高达99%，在系统进化树中与其亲缘关系也最近。该种在国内首次报道和描述，为木霉属中国新记录种。

关键词: ITS序列分析, 近深绿木霉, tef1-α序列分析, 中国新记录种

Abstract: Trichoderma strain Y15 was isolated from the vegetable soil under Shandong greenhouse, and identified by analysis of internal transcribed spacer regions of the rRNA gene cluster (ITS), partial sequences of transcription extensions factor 1alpha (tef1α) and morphological characteristics. Results show that Y15 is T. paratroviride. The strain is colony dense, panniform, greyishgreen on PDA. Conidiophore main axes is long with short secondary branches, not extensively rebranching. Its branches are coupled or verticillate. Phialides usually has 2~4 verticils, lageniform or subulate, straight or flexuous. Conidia is subglobose to obovoid, greenish and smooth. The tef1α sequence of strain Y15 has 99% homology with strains S489 and S385 of T. paratroviride type, and has the closest genetic relationship with them in phylogenetic tree. It is originally reported in China as a new Chinese record of genus Trichoderma.

Key words: Trichoderma paratroviride, ITS, tef1-α, Chinese new record

中图分类号:

Q949.32

张广志, 张新建, 陈凯, 吴晓青,李纪顺，杨合同. 木霉属中国新记录种Trichoderma paratroviride[J]. 山东科学, 2015, 28(5): 35-40.

ZHANG Guangzhi, ZHANG Xinjian,CHEN Kai,WU Xiaoqing,LI Jishun,YANG Hetong. Trichoderma paratroviride,Chinese new record of Trichoderma[J]. SHANDONG SCIENCE, 2015, 28(5): 35-40.

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木霉属中国新记录种Trichoderma paratroviride

Trichoderma paratroviride,Chinese new record of Trichoderma

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