哈茨木霉LTR-2对蔬菜种子和幼苗耐盐性的影响及其作用机制

doi:10.3976/j.issn.1002-4026.2015.05.005

山东科学 ›› 2015, Vol. 28 ›› Issue (5): 27-34.doi: 10.3976/j.issn.1002-4026.2015.05.005

哈茨木霉LTR-2对蔬菜种子和幼苗耐盐性的影响及其作用机制

赵忠娟1，扈进冬1，吴晓青1，赵晓燕1，李哲1，李纪顺1，杨合同1, 2*

1．山东省科学院生物技术研究中心，山东省应用微生物重点实验室，山东济南 250014； 2．山东理工大学生命科学学院，山东淄博 255049

收稿日期:2015-05-20 出版日期:2015-10-20 发布日期:2015-10-20
作者简介:赵忠娟(1986- )，女，助理研究员，研究方向为耐盐植物选育栽培及耐盐机制。Email: zzjfrances@aliyun.com
基金资助:
山东省科学院青年科学基金(2014QN017)；山东省自然科学基金（ZR2015PC009）

Impact and mechanism of Trichoderma harzianum strain LTR-2 on salt tolerance of vegetable seeds and seedlings

ZHAO Zhongjuan1, HU Jindong1, WU Xiaoqing1, ZHAO Xiaoyan1,LI Zhe1, LI Jishun1, YANG Hetong1, 2*

1.Shandong Provincial Key Laboratory of Applied Microbiology, Biotechnology Center, Shandong Academy of Sciences, Jinan 250014, China;2.School of Life Sciences, Shandong University of Technology, Zibo 250049, China

Received:2015-05-20 Online:2015-10-20 Published:2015-10-20

摘要/Abstract

摘要： 研究了盐胁迫条件下哈茨木霉LTR2处理对黄瓜、萝卜和番茄3种蔬菜的种子萌发、幼苗生长和生理生化反应的影响。结果表明，盐胁迫条件下，LTR-2处理的蔬菜种子萌发率明显高于未处理；LTR-2处理的蔬菜幼苗的株高、鲜重、叶片中叶绿素含量、净光合作用速率（Pn值）以及抗氧化酶活性明显高于未处理，而膜质损伤（相对电导率和丙二醛含量）弱于未处理的幼苗。哈茨木霉LTR-2能够促进盐胁迫下蔬菜种子的萌发以及幼苗的生长和光合作用，提高幼苗的抗氧化能力并降低膜质损伤，增强幼苗对盐胁迫的耐受性。

关键词: 哈茨木霉, 蔬菜种子, 盐胁迫, 耐盐性

Abstract: We addressed the impact of Trichoderma harzianum strain LTR-2 on seed germination, seedling growth and physiological and biochemical response of cucumber, radish and tomato under salinity stress. Results show that germination rate of vegetable seeds treated with LTR-2 is significantly higher than that of seeds untreated with LTR-2 under NaCl stress. Shoot length, fresh weight, chlorophyll content, net photosynthetic rate (Pn value) and antioxidant enzyme activity of vegetable seedlings treated with LTR-2 are significantly higher than those of seedlings without LTR-2 treatment. However, relative conductivity and MDA content are less than those without LTR-2 treatment. This indicates that T. harzianum strain LTR-2 can stimulate germination of vegetable seeds, growth and photosynthesis of seedlings, improve antioxidant capacity of seedlings, reduce membrane damage, and enhance salt tolerance of seedlings.

Key words: vegetable seeds, Trichoderma harzianum, salinity stress, salt tolerance

中图分类号:

S182

赵忠娟，扈进冬，吴晓青，赵晓燕，李哲，李纪顺，杨合同. 哈茨木霉LTR-2对蔬菜种子和幼苗耐盐性的影响及其作用机制[J]. 山东科学, 2015, 28(5): 27-34.

ZHAO Zhongjuan, HU Jindong, WU Xiaoqing, ZHAO Xiaoyan,LI Zhe, LI Jishun, YANG Hetong. Impact and mechanism of Trichoderma harzianum strain LTR-2 on salt tolerance of vegetable seeds and seedlings[J]. SHANDONG SCIENCE, 2015, 28(5): 27-34.

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哈茨木霉LTR-2对蔬菜种子和幼苗耐盐性的影响及其作用机制

Impact and mechanism of Trichoderma harzianum strain LTR-2 on salt tolerance of vegetable seeds and seedlings

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