J4 ›› 2011, Vol. 24 ›› Issue (2): 28-34.

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Isolation and identification of a carbendazim-degrading strain and its degrading characteristics

 HUANG Yu-Jie, ZHANG Xin-Jian, REN Yan, LI Ji-Shun, ZHANG Guang-Zhi, YANG He-Tong   

  1. handong Provincial Key Laboratory of Applied Microbiology,Biotechnology Center, Shandong Academy of Sciences, Jinan 250014, China
  • Received:2011-01-05 Online:2011-04-20 Published:2011-04-20

Abstract:

       We obtained a strain dj1-11 from carbendazimcontaminated soil, which had better carbendazim degradation function. It was identified as Rhodococcus erythropolis through physiological and biochemical identification and contrast analysis of 16S rDNA sequences. Experimental results show that trace nitrogen source adding can improve carbendazim degradation. Carbendazim can serve as its unique carbon source and degradation rate can reach 99.15% under the condition of incubation in mineral salt medium of 1000 μg/mL carbendazim , temperature 28℃ and 48h shake bed.It can effectively degrade carbendazim at the pH value of 4~9 and temperature 20~30℃ (optimal degradation temperature).

Key words: carbendazim, biodegradation, Rhodococcus erythropolis, degrading characteristics

CLC Number: 

  • X172